UJI POTENSI Bacillus sp. DAN Escherichia coli DALAM MENGHASILKAN INDOLE ACETIC ACID (IAA) TANPA MENGGUNAKAN TRIPTOFAN PADA MEDIA PERTUMBUHAN
Indole Acetic Acid (IAA) is the important phytohormone for boosting plantgrowth
promotion. It is related with amino acid tryptophan as a precursor for
building IAA. However some bacteria such as Bacillus sp. and Escherichia
coli enable to produce IAA without L-tryptophan on culture medium via the
tryptophan independent pathways. This study aims to determine the potential
of indigenous Bacillus sp. and E. coli isolate in producing IAA via Trpindependent
pathways (without L- tryptophan). The stage of this study
consists of growing of isolate on liquid media without tryptophan and
measuring the levels of IAA using Salkowsky method. Bacillus sp and
Escherichia coli could produce IAA with the concentration of 0,0236 mmol/L
and 0,024 mmol/L, respectively. The concentration of IAA produced by these
bacteria is quite low because of their ability to synthesis IAA from the
independent tryptophan pathway. The independent tryptophan pathway
means these bacteria could synthesize IAA without using tryptophan as a
precursor on their growth medium.
URI
https://repo.itera.ac.id/depan/submission/SB1802240009
Keyword
Bacillus sp Escherichia coli IAA tryptophan