Assay of Antagonistic Bacteria of Single Isolate and Combination to Control Seedling-off in Chili Seed caused by Fusarium oxysporum
Single isolate and combination of chitinolytic and non-chitinolytic bacteria
were assayed to know their ability in reducing chili seedling-off caused by Fusarium
oxysporum. Eight antagonistic bacterial isolates i.e. Bacillus sp. BK13, Alcaligenes sp.
BK14, Enterobacter sp. BK15, Citrobacter sp. BK16, and Bacillus sp. BK17 showing
chitinolytic activity, and Pseudomonas sp. KM01, Alcaligenes sp. KM02, and Serratia
sp. KM04 with no chitinolytic activity were assayed to inhibit F. oxysporum and Candida
albicans growth in vitro. All isolates showed varied ability in inhibiting the fungal
growth, in which Enterobacter sp. BK15 and Bacillus sp. BK13 inhibited the most.
Inhibition assay on C. albicans indicated that non-chitinolytic bacterial isolates is likely
to produce other toxic metabolic compounds. Single isolate of Enterobacter sp. BK15 and
combination of Bacillus sp. BK13+Serratia sp. KM04 as well showed to reduce more
chili seedling-off. All treatments produce higher seedling height and seedling dry-weight
than that of isolate-free seeds planted in fungus-inoculated soil but one, Enterobacter sp.
BK15+Pseudomonas sp. KM01.
Journal of Pure and Applied Microbiology